Journal: Aging (Albany NY)
Article Title: CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
doi: 10.18632/aging.205851
Figure Lengend Snippet: CRYAB positively regulates the osteogenic differentiation of BMSCs. ( A ) mRNA expression level of CRYAB in osteoporosis samples was detected using qRT-PCR. ( B , C ) mRNA expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using qRT-PCR (n=3). ( D , E ) Protein expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using the Western blotting method (n=3). ( F ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.
Article Snippet: The information of antibodies was as follows: anti-OCN antibody (Proteintech, 23418-1-AP), OPN antibody (Proteintech, 22952-1-AP), RUNX2 (Abcam, UK, ab192256), COLI antibody (Proteintech, 14695-1-AP), CRYAB antibody (Proteintech, 15808-1-AP), FTH1 antibody (Cell Signaling Technology, 4393) and anti-GAPDH antibody (Abcam, ab8245).
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Activity Assay, ALP Assay