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Corning Life Sciences collagen type i coli
Collagen Type I Coli, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+i+coli/collagen+type+i+coli/pm39356134-64-4-11
Average 90 stars, based on 1 article reviews
collagen type i coli - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Biocompatible nHA/Col-BMP-9/GM Scaffold: Synthesis, Characterization, and Effects on Bone Marrow Mesenchymal Stem Cells
Article Snippet: nHA (Emperor Nano Material Co., Ltd, Nanjing, China) and collagen I (ColI; Corning Inc., Corning, NY, USA) were chosen as the basic matrices for synthesis of the nHAC-B9/GM composite scaffold.



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Sequences of siRNAs and primers in the present study.

Journal: Aging (Albany NY)

Article Title: CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1

doi: 10.18632/aging.205851

Figure Lengend Snippet: Sequences of siRNAs and primers in the present study.

Article Snippet: The information of antibodies was as follows: anti-OCN antibody (Proteintech, 23418-1-AP), OPN antibody (Proteintech, 22952-1-AP), RUNX2 (Abcam, UK, ab192256), COLI antibody (Proteintech, 14695-1-AP), CRYAB antibody (Proteintech, 15808-1-AP), FTH1 antibody (Cell Signaling Technology, 4393) and anti-GAPDH antibody (Abcam, ab8245).

Techniques:

CRYAB positively regulates the osteogenic differentiation of BMSCs. ( A ) mRNA expression level of CRYAB in osteoporosis samples was detected using qRT-PCR. ( B , C ) mRNA expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using qRT-PCR (n=3). ( D , E ) Protein expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using the Western blotting method (n=3). ( F ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Journal: Aging (Albany NY)

Article Title: CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1

doi: 10.18632/aging.205851

Figure Lengend Snippet: CRYAB positively regulates the osteogenic differentiation of BMSCs. ( A ) mRNA expression level of CRYAB in osteoporosis samples was detected using qRT-PCR. ( B , C ) mRNA expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using qRT-PCR (n=3). ( D , E ) Protein expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using the Western blotting method (n=3). ( F ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Article Snippet: The information of antibodies was as follows: anti-OCN antibody (Proteintech, 23418-1-AP), OPN antibody (Proteintech, 22952-1-AP), RUNX2 (Abcam, UK, ab192256), COLI antibody (Proteintech, 14695-1-AP), CRYAB antibody (Proteintech, 15808-1-AP), FTH1 antibody (Cell Signaling Technology, 4393) and anti-GAPDH antibody (Abcam, ab8245).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Activity Assay, ALP Assay

Knockdown of FTH1 induces ferroptosis and suppresses osteogenic differentiation of BMSCs. ( A ) The efficiency of FTH1 knockdown was validated using Western blotting method (n=3). ( B , C ) Cellular Fe and ROS levels were detected after FTH1 knockdown in BMSCs (n=3). ( D , E ) The cell viabilities were detected using CCK-8 assay (n=3). ( F ) mRNA expression levels of OCN, OPN, RUNX2, and COLI when FTH1 was silenced were detected using qRT-PCR (n=3). ( G ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). ns: no significance; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Journal: Aging (Albany NY)

Article Title: CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1

doi: 10.18632/aging.205851

Figure Lengend Snippet: Knockdown of FTH1 induces ferroptosis and suppresses osteogenic differentiation of BMSCs. ( A ) The efficiency of FTH1 knockdown was validated using Western blotting method (n=3). ( B , C ) Cellular Fe and ROS levels were detected after FTH1 knockdown in BMSCs (n=3). ( D , E ) The cell viabilities were detected using CCK-8 assay (n=3). ( F ) mRNA expression levels of OCN, OPN, RUNX2, and COLI when FTH1 was silenced were detected using qRT-PCR (n=3). ( G ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). ns: no significance; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Article Snippet: The information of antibodies was as follows: anti-OCN antibody (Proteintech, 23418-1-AP), OPN antibody (Proteintech, 22952-1-AP), RUNX2 (Abcam, UK, ab192256), COLI antibody (Proteintech, 14695-1-AP), CRYAB antibody (Proteintech, 15808-1-AP), FTH1 antibody (Cell Signaling Technology, 4393) and anti-GAPDH antibody (Abcam, ab8245).

Techniques: Knockdown, Western Blot, CCK-8 Assay, Expressing, Quantitative RT-PCR, Activity Assay, ALP Assay

CRYAB regulates ferroptosis and osteogenic differentiation of BMSCs in an FTH1-dependent manner. ( A ) The protein levels of CRYAB and FTH1 were detected using a Western blotting assay (n=3). ( B – D ) Cellular Fe and ROS levels and cell viability were detected (n=3). ( E ) mRNA expression levels of OCN, OPN, RUNX2, and COLI were detected using qRT-PCR (n=3). ( F ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Journal: Aging (Albany NY)

Article Title: CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1

doi: 10.18632/aging.205851

Figure Lengend Snippet: CRYAB regulates ferroptosis and osteogenic differentiation of BMSCs in an FTH1-dependent manner. ( A ) The protein levels of CRYAB and FTH1 were detected using a Western blotting assay (n=3). ( B – D ) Cellular Fe and ROS levels and cell viability were detected (n=3). ( E ) mRNA expression levels of OCN, OPN, RUNX2, and COLI were detected using qRT-PCR (n=3). ( F ) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.

Article Snippet: The information of antibodies was as follows: anti-OCN antibody (Proteintech, 23418-1-AP), OPN antibody (Proteintech, 22952-1-AP), RUNX2 (Abcam, UK, ab192256), COLI antibody (Proteintech, 14695-1-AP), CRYAB antibody (Proteintech, 15808-1-AP), FTH1 antibody (Cell Signaling Technology, 4393) and anti-GAPDH antibody (Abcam, ab8245).

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Activity Assay, ALP Assay